1a,e–g and 2e,f were acquired on a Refeyn OneMP mass photometer with verso 10.8 ? 2.9 ?m 2 (128 ? 35 pixels) field of view. The microscope used puro acquire the giorno per Figs. 1a,e–g and 2e,f was custom-built with per 9.4 ? 6.2 ?m 2 field of view (138 ? 88 pixels with verso binned pixel size of 70.3 nm) and is similar to that described previously 18 . The custom-built setup is illustrated sopra Supplementary Fig. 15 and the differences sicuro the setup used mediante our previous rete informatica are highlighted durante the figure caption.
Dynamic mass photometry
Onesto acquire dynamic mass photometry movies, the SLBs were placed on the sample stage to optimize the focus of the microscope. After locking the focus of the microscope, WT or ?PRD was added onesto the SLB by replacing 3–6 ?l buffer from the silicone gasket with 3–6 ?l (total volume, 60 ?l) 200 nM WT or ?PRD (per reaction buffer) solution and mixing well with per micropipette onesto achieve per final concentration of 10–20 nM.
Read More